Principle of CNOOC biotechnology eosin methylene blue agar identification medium

  Eosin methylene blue agar medium (EMB) Chinese marine eosin methylene blue agar medium is a weakly selective medium for the separation of intestinal pathogens
It is mainly used for the separation and identification of intestinal pathogens, especially coliforms
1300-1 ⑨ 30-979 the formula of the medium contains peptone, lactose, Eosin Y, methylene blue, agar powder, beef extract powder and sodium chloride
Eosin Y is an acidic dye and methylene blue is an alkaline dye, which can be used as a pH indicator; As bacteriostatic agents, eosin and methylene blue can inhibit the growth of Gram-positive bacteria to a certain extent, but they have no inhibitory effect on E
coli zhzbio Com can also be used to distinguish the fermentation of lactose
Fermented lactose will produce acid
Under acidic conditions, E
coli will form purple black colonies or colorless and transparent colonies with black centers on the periphery
However, for lactose that does not produce acid, eosin and methylene blue cannot be combined
Therefore, if Salmonella and Shigella are present in microorganisms, colorless colonies will be formed
This medium can identify different strains of intestinal pathogenic bacteria according to the color of colonies

[application field]
Scientific research, experiment and biology

[usage]
Weigh 42
0g of eosin methylene blue agar medium, add 1L of water for injection, heat and boil until completely dissolved, then repack, and autoclave at 116 ℃ for 30 minutes

[precautions]
1
Please operate in a clean environment to avoid the interference of miscellaneous bacteria

2
During operation, please wear disposable masks, gloves and other protective tools to avoid skin contact

[storage conditions]
This product is easy to absorb moisture and needs to be kept away from moisture and light
It should be sealed and stored in a cool and dry place