Components of bile sulfur emulsion agar medium of CNOOC Biotechnology

  Bile sulfur milk agar medium DHL China Marine bile sulfur milk agar medium is often used for the separation of Escherichia coli, Salmonella and Shigella
The main components are peptone, beef extract powder, lactose, sucrose, sodium deoxycholate, sodium thiosulfate, sodium citrate, ammonium ferric citrate, neutral red and agar powder

  Among them, peptone and beef extract powder provide the required carbon source, nitrogen source, vitamins and minerals; Lactose and sucrose are fermentable sugars with decomposition ability
1300-1 ⑨ 30-979 colonies are opaque red, indicating that bacteria have been decomposed by lactose and sucrose
Colonies are colorless and transparent, indicating that lactose and sucrose do not decompose bacteria, so as to identify pathogens; Sodium deoxycholate and sodium citrate can inhibit the development of Gram-positive bacteria, most coliforms and Proteus, but they have little selectivity for Gram-negative bacteria, so they do not affect the growth of Salmonella; Sodium thiosulfate and ammonium ferric citrate are used to detect the production of hydrogen sulfide zhzbio The bacteria produced by hydrogen sulfide in com will make the colony center black; Neutral red is a pH indicator with a pH value of 7
0 ~ 7
4; Agar is the coagulant of DHL medium in solid form, so the medium also belongs to solid medium

[application field]
Biology and scientific research experiment

[usage]
Instructions for bile sulfur emulsion agar medium
[precautions]
1
Wear gloves, masks and other protective tools before the experiment to avoid contact with skin

2
Please operate in a clean environment to avoid the interference of miscellaneous bacteria

[storage conditions]
This product is easy to absorb moisture
After use, it is placed in a dark, cool and dry place and sealed for storage