Composition and application of CNOOC biotechnology trisaccharide iron agar medium

  Trisaccharide iron agar medium TSI trisaccharide iron agar medium is a kind of bacterial medium, which can identify and diagnose various genera of Enterobacteriaceae
It has the ability to ferment the types of sugars and produce hydrogen sulfide
1300-1 ⑨ 30-979 China Marine Biotechnology trisaccharide iron agar medium contains three sugars, namely lactose, sucrose and glucose
Through the utilization of different sugars, Different bacteria have different performances in bottom acid production, slope acid production, hydrogen sulfide production and gas production

  First of all, glucose with the least content will produce a small amount of acid after contact and utilization by bacteria
Bacteria that can only use glucose will carry out aerobic respiration on the slope and be oxidized into carbon dioxide and water, so it is impossible to significantly change the pH color
However, bacteria will use peptone containing nitrogen source material in the culture medium to produce alkaline products to make the slope alkaline, Red deepened

  Secondly, after glucose depletion, the bacteria will use lactose and sucrose
Due to the high content of lactose and sucrose, a large amount of acid will be produced after fermentation, so that the color of the whole medium indicator turns yellow
In addition, black precipitation occurs in the culture because some bacteria will use sodium thiosulfate to produce hydrogen sulfide
Hydrogen sulfide meets iron to form ferrous sulfide, making the colony black

  The results show that there is black precipitation on the TSI culture baseband, indicating the production of hydrogen sulfide, and the Yellow Medium indicates that bacteria ferment lactose or sucrose
The slope of bacteria that can only use glucose turns red
Because the bottom is anaerobic and acids are not oxidized, the bottom is still yellow

  Zhonghai trisaccharide iron agar medium zhzbio Com affects the pH through the above acid production of lactose, sucrose and glucose, and makes the bacteria color differently, so as to identify and diagnose the members of various genera of Enterobacteriaceae

[application field]
Biology and scientific research experiment

[usage]
Instructions for trisaccharide iron agar medium
[precautions]
1
Wear gloves, masks and other protective tools before the experiment to avoid contact with skin

2
Please operate in a clean environment to avoid the interference of miscellaneous bacteria

[storage conditions]
This product is easy to absorb moisture
It should be sealed and stored in a cool and dry place after use