Manufacturer of cardiac troponin I detection reagent Shanghai Kaichuang biological

Cardiac troponin I (cTnI) is a subunit of cardiac troponin complex, which exists on the fine filaments of myocardial myofibrils and is an important structural protein of cardiomyocytes
CTnI has a unique amino acid sequence and a molecular weight of about 24 kDa
It is tissue-specific and is a unique substance in myocardium
Compared with skeletal muscle troponin I, cTnI has 31 additional amino acids at the N-terminal
When the myocardial cell membrane is intact, cTnI can not enter the blood circulation through the cell membrane
When cardiomyocytes become denatured and necrotic due to ischemia and hypoxia, cTnI can diffuse into the intercellular matrix through the damaged cell membrane and return to the blood through lymph
Serum cTnI increased at an average of 4 ~ 6 hours after the onset of chest pain in acute myocardial infarction (AMI), with a peak time of 12 ~ 24 hours, and returned to normal after 7 ~ 10 days
In some patients with AMI, the serum level can be as high as 1300ng / ml
The average level of cTnI in normal blood is only below 0
06ng/ml
CTnI has the characteristics of early occurrence in blood, high sensitivity, strong specificity and long diagnostic window
It is an ideal index for the diagnosis of AMI This product uses colloidal gold immunochromatography to qualitatively detect cTnI in human serum / plasma based on the principle of double antibody sandwich method
On the fiber membrane in the reagent card, the quality control line (line C) is coated with Sheep anti mouse IgG, the detection line (line T) is coated with anti cTnI single * * antibody-2, and the other end is coated with anti cTnI single * * antibody-1-colloidal gold complex
During detection, the tested serum / plasma sample is first mixed with anti cTnI single * * anti-1-colloidal gold, and then chromatographed upward along the fiber membrane through t line and C line in turn
If cTnI exists in serum / plasma, cTnI first binds to anti cTnI single * * antibody-1-colloidal gold to form & ldquo; CTnI anti cTnI monoclonal antibody-1-colloidal gold complex;, When chromatography to the T-line, it will be captured by another anti cTnI single * * antibody-2 pre coated on the T-line, and a red line is formed at the T-line, which is a positive result
If there is no cTnI in serum / plasma, no line will be formed at the T line, which is a negative result
No matter whether there is cTnI in the serum / plasma sample or not, there should always be a red line at line C
The appearance of line C indicates that the kit is effective and operated correctly
Cardiac troponin I test reagent products www
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