CNOOC Brucella competitive ELISA antibody detection kit

  Brucella competitive ELISA antibody detection kit Brucella competitive ELISA antibody detection kit uses competitive ELISA method to pre coat Brucella antigen 1300-1 ⑨ 30-979 on the micropore of enzyme label plate
In the experiment, add diluted serum to be tested, enzyme label secondary antibody working solution and single * * antibody working solution
After incubation, if the sample contains brucella antibody, Then it competes with the single * * antibody for the coated antigen epitope in the plate hole, and the enzyme labeled secondary antibody combines with the single * * antibody to form the Brucella antigen single * * antibody enzyme labeled secondary antibody complex
After incubation, the excess free antibody is removed by washing, TMB chromogenic solution is added into the micropore, the blue signal produced by enzyme catalysis is inversely proportional to the antibody content in the sample, and the termination solution is added to terminate the reaction, The absorbance OD value in the reaction well was determined by enzyme labeling instrument at 450 nm wavelength

[product features]
CNOOC biotechnology Brucella competitive ELISA antibody detection kit zhzbio COM is a Brucella antigen test diagnostic kit pre coated on the micropores of enzyme labeled plate
Compared with the traditional complement binding test diagnostic kit, this kit has the characteristics of high sensitivity, easy to use, fast, high throughput and high degree of standardization
Compared with the traditional ELISA diagnostic kit, it has the characteristics of high specificity and can detect various Brucella specific antibodies, It is an ideal new diagnostic tool for brucellosis

[precautions]
1
Before using the kit, all reagents should be balanced to room temperature
Store it at 2 ~ 8 ℃ after use

2
Kit components of different batches shall not be mixed, and cross slot staining shall be prevented when different reagents are used

3
The substrate solution and termination solution shall not be exposed to strong light or contact with oxidant

4
When the amount of serum to be tested is large, all serum to be tested shall be diluted first, and then added to the antigen coating plate to make the reaction time consistent

5
Dilution 10 & times; When concentrating the washing solution, if crystals are found, they should be heated to dissolve before use

6
When pipetting, it shall be as accurate as possible to prevent bubbles

7
The time and temperature specified in each operation step shall be strictly observed

[storage conditions]
Keep away from light at 2 ~ 8 ℃